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Research Template

Experimental Design Template

Use this template to design rigorous, reproducible research studies

1. Study Information

Study Title:

[Enter study title]

Principal Investigator:

[Name, credentials]

Study Dates:

[Start date] - [End date]

IRB Protocol Number:

[IRB #]

2. Research Question & Hypothesis

Primary Research Question

[State your primary research question]

Hypothesis

H0: [Null hypothesis]
H1: [Alternative hypothesis]

Secondary Objectives

• [Secondary objective 1]
• [Secondary objective 2]
• [Secondary objective 3]

3. Study Design

Study Type: [ ] In vitro [ ] Ex vivo [ ] Clinical [ ] Observational

Experimental Groups:

Control Group: [Description]
Treatment Group 1: [Description]
Treatment Group 2: [Description]

Sample Size Calculation:

Power: [e.g., 80%]
Alpha: [e.g., 0.05]
Effect size: [Expected difference]
Required n per group: [Number]

4. Methods

4.1 Materials

Cell lines: [Specify]
Reagents: [List with catalog numbers]
Equipment: [List]

4.2 Procedures

Step 1: [Detailed procedure]
Step 2: [Detailed procedure]
Step 3: [Detailed procedure]

4.3 Outcome Measures

Primary endpoint: [What you're measuring]
Secondary endpoints: [Additional measurements]
Timepoints: [When measurements occur]

5. Data Analysis Plan

Statistical Tests:

[Specify tests for each comparison]

Software:

[Prism, R, Python, etc.]

Significance Level: α = 0.05 (two-tailed)

6. Timeline

Week 1-2: [Activity]
Week 3-4: [Activity]
Week 5-6: [Activity]
Week 7-8: [Data analysis]

7. References

[1] Citation
[2] Citation
[3] Citation